Sains Malaysiana 53(1)(2024): 87-98
http://doi.org/10.17576/jsm-2024-5301-07
Development of Semolina Starch/Agar-Based Intelligent Films by
Incorporating Butterfly Pea Flower Anthocyanins to
Monitor the Freshness of Prawns
(Pembangunan Filem Pintar Berasaskan Kanji Semolina/Agar dengan
Menggabungkan Antosianin Bunga Telang untuk Memantau Kesegaran Udang)
SRISANTHIYA SARAVANAN1, NOR ADILAH
ABDULLAH1, NUR NABILAH HASANAH1, EZZAT MOHAMAD AZMAN1,
NUR HANANI ZAINAL ABEDIN1 & MOHAMMAD RASHEDI ISMAIL-FITRY1,2,*
1Department of Food Technology, Faculty of Food
Science and Technology, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia
2Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia
Diserahkan: 13 Oktober 2023/Diterima: 29 Disember 2023
Abstract
The natural plant-derived pH indicator film which monitors food freshness
during storage has gained attention among researchers due to consumer awareness
of food quality, freshness, and food safety. This study aims to develop a
semolina/agar-based film (S/A) incorporated with anthocyanins from butterfly pea flower extract (BPE) as a freshness indicator for tiger
prawns and freshwater prawn during 4 days of storage at 4±1 °C. The
semolina/agar-based butterfly pea extract (S/A/BPE) films were developed with different
concentrations of BPE (3, 6, and 9%) by casting method. Increasing BPE up to 9%
in the S/A film increased the thickness and moisture content, lowering the
films’ water permeability. The film also had a smoother surface structure with
a darker colour. Different pH solutions (pH 2.0-12.0) also changed the colour
of films, depending on the BPE concentrations. The application of S/A/BPE films
on tiger prawns and freshwater prawns caused colour changes from dark blue at
day 0 (fresh stage) to intense green at day 2 (beginning of spoilage)
indicating the spoilage of samples. The pH values of both prawns were gradually
increased. However, no major changes were observed in the texture profile
analysis. These results indicate that S/A/BPE film has the potential to be used
as a pH indicator film to detect the freshness of prawn samples.
Keywords: Butterfly pea extract; freshwater prawns; pH indicator film;
semolina; tiger prawns
Abstrak
Filem penunjuk pH daripada tumbuhan semula jadi yang memantau kesegaran makanan semasa penyimpanan telah mendapat perhatian dalam kalangan penyelidik kerana kesedaran pengguna tentang kualiti makanan, kesegaran dan keselamatan makanan. Kajian ini bertujuan untuk menghasilkan filem berasaskan semolina/agar (S/A) yang digabungkan dengan antosianin daripada ekstrak bunga telang (BPE) sebagai penunjuk kesegaran udang harimau dan udang galah selama 4 hari penyimpanan pada suhu 4±1 °C. Filem ekstrak bunga telang berasaskan semolina/agar (S/A/BPE) telah dihasilkan dengan kepekatan BPE (3, 6 dan 9%) yang berbeza dengan kaedah tuangan. Peningkatan BPE sehingga 9% dalam filem S/A meningkatkan ketebalan dan kandungan lembapan, menurunkan kebolehtelapan air bagi filem. Filem ini juga mempunyai struktur permukaan yang lebih halus dengan warna yang lebih gelap. Larutan pH yang berbeza (pH
2.0-12.0) juga mengubah warna filem, bergantung pada kepekatan BPE. Penggunaan filem S/A/BPE pada udang harimau dan udang galah menyebabkan perubahan warna daripada biru tua pada hari 0 (segar) kepada hijau pekat pada hari ke-2 (permulaan kerosakan) yang menunjukkan kerosakan sampel. Nilai pH kedua-dua udang telah meningkat secara beransur-ansur. Walau bagaimanapun, tiada perubahan besar diperhatikan dalam analisis profil tekstur. Keputusan ini menunjukkan bahawa filem S/A/BPE berpotensi untuk digunakan sebagai filem penunjuk pH untuk mengesan kesegaran sampel udang.
Kata kunci: Ekstrak bunga telang; penunjuk pH; semolina; udang galah; udang harimau
RUJUKAN
Alizadeh-Sani, M., Mohammadian, E., Rhim, J.W. &
Jafari, S.M. 2020. pH-sensitive (halochromic) smart packaging films based on
natural food colorants for the monitoring of food quality and safety. Trends
in Food Science & Technology 105: 93-144.
Bhargava, N., Singh S.V., Mor, R.S. & Kumar, K. 2020.
Active and intelligent biodegradable packaging films using food and food
waste-derived bioactive compounds: A review. Trends in Food Science &
Technology 105: 385-401.
Boonsiriwit, A., Lee, M., Kim, M., Inthamat, P.,
Siripatrawan, U. & Lee, Y.S. 2021. Hydroxypropyl
methylcellulose/microcrystalline cellulose biocomposite film incorporated with
butterfly pea anthocyanin as a sustainable pH-responsive indicator for
intelligent food-packaging applications. Food Bioscience 44: 101392.
Chawla, R., Sivakumar, S. & Kaur, H. 2021.
Antimicrobial edible films in food packaging: Current scenario and recent
nanotechnological advancements - A review. Carbohydrate Polymer Technologies
and Applications 2: 100024.
Chen, S., Wu, M., Lu, P., Gao, L., Yan, S. & Wang, S.
2020. Development of pH indicator and antimicrobial cellulose nanofibre
packaging film based on purple sweet potato anthocyanin and oregano essential
oil. International Journal of Biological Macromolecules 149: 271-280.
Choi, I., Lee, J.Y., Lacroix, M. & Han, J. 2017.
Intelligent pH indicator film composed of agar/potato starch and anthocyanin
extracts from purple sweet potato. Food Chemistry 218: 122-128.
Gokoglu, N., Gumus, B., Ceylan, A. & Gokoglu, M. 2022.
Storage in ice incorporated antimelanotic agent and its effects on melanosis
and quality of giant red shrimp (Aristaeomorpha foliacea). Food
Bioscience 46: 101599.
Guo, Y., Zhang, B., Zhai, S., Qiao, D. & Xie, F. 2021.
Plasticized starch/agar composite films: Processing, morphology, structure,
mechanical properties and surface hydrophilicity. Coatings 11(3): 311.
Halász, K. & Csóka, L. 2018. Black chokeberry (Aronia
melanocarpa) pomace extract immobilized in chitosan for colorimetric pH
indicator film application. Food Packaging and Shelf Life 16: 185-193.
Hasanah, N.N., Mohamad Azman, E., Rozzamri, A., Zainal
Abedin, N.H. & Ismail-Fitry, M.R. 2023. A Systematic review of butterfly
pea flower (Clitoria ternatea L.): Extraction and application as a food
freshness pH-Indicator for polymer-based intelligent packaging. Polymers 15(11): 2541.
Hashim, S.B.H., Elrasheid Tahir, H., Liu, L., Zhang, J.,
Zhai, X., Ali Mahdi, A., Nureldin Awad, F., Hassan, M.M., Xiaobo, Z. &
Jiyong, S. 2022. Intelligent colorimetric pH sensoring packaging films based on
sugarcane wax/agar integrated with butterfly pea flower extract for optical
tracking of shrimp freshness. Food Chemistry 373: 131514.
Husin, N., Rahim, M.Z.A., Noor, M.A.M., Rashedi,
I.F.M. & Hassan, N. 2020. Real-time monitoring of food freshness using delphinidin-based visual indicator. Malaysian
Journal of Analytical Sciences 24(4): 558-569.
Jafarzadeh, S., Alias, A.K., Ariffin, F. & Mahmud, S.
2018. Physico-mechanical and microstructural properties of semolina flour films
as influenced by different sorbitol/glycerol concentrations. International
Journal of Food Properties 21(1): 983-995.
Jafarzadeh, S., Ariffin, F., Mahmud, S., Alias, A.K.,
Najafi, A. & Ahmad, M. 2017. Characterization of semolina biopolymer films
enriched with zinc oxide nano rods. Italian Journal of Food Science 29(2):
195-208.
Kang, S., Wang, H., Xia, L., Chen, M., Li, L., Cheng, J.,
Li, X. & Jiang, S. 2020. Colorimetric film based on polyvinyl alcohol/okra
mucilage polysaccharide incorporated with rose anthocyanins for shrimp
freshness monitoring. Carbohydrate Polymers 229: 115402.
Kim, H-J., Roy, S. & Rhim, J.W. 2022. Gelatin/agar-based
color-indicator film integrated with Clitoria ternatea flower
anthocyanin and zinc oxide nanoparticles for monitoring freshness of shrimp. Food
Hydrocolloids 124: 107294.
Koshy, R.R., Reghunadhan, A., Mary, S.K., Pillai, P.S.,
Joseph, S. & Pothen, L.A. 2022. pH indicator films fabricated from soy
protein isolate modified with chitin nanowhisker and Clitoria ternatea flower extract. Current Research in Food Science 5: 743-751.
Lam, X.M., Abdorreza, M.N., Farzaneh, G., Azhar, M.E.,
Shima, J. & Al-Hassan, A.A. 2020. Characterization of pH sensitive sago
starch films enriched with anthocyanin-rich torch ginger extract. International
Journal of Biological Macromolecules 164: 4603-4612.
Lin, D., Sun, L.C., Chen, Y.L., Liu, G.M., Miao, S. &
Cao, M.J. 2022. Shrimp spoilage mechanisms and functional films/coatings used
to maintain and monitor its quality during storage. Trends in Food Science
& Technology 129: 25-37.
Mary, S.K., Koshy, R.R., Daniel, J., Koshy, J.T., Pothen,
L.A. & Thomas, S. 2020. Development of starch based intelligent films by
incorporating anthocyanins of butterfly pea flower and TiO2 and
their applicability as freshness sensors for prawns during storage. RSC
Advances: 10(65): 39822-39830.
Merz, B., Capello, C., Leandro, G.C., Moritz, D.E.,
Monteiro, A.R. & Valencia, G.A. 2020. A novel colorimetric indicator film
based on chitosan, polyvinyl alcohol and anthocyanins from jambolan (Syzygium
cumini) fruit for monitoring shrimp freshness. International Journal of
Biological Macromolecules 153: 625-632.
Mohammadalinejhad, S., Almasi, H. & Moradi, M. 2020.
Immobilization of Echium amoenum anthocyanins into bacterial cellulose
film: A novel colorimetric pH indicator for freshness/spoilage monitoring of
shrimp. Food Control 113: 107169.
Mostafavi, F.S. & Zaeim, D. 2020. Agar-based edible
films for food packaging applications - A review. International Journal of
Biological Macromolecules 159: 1165-1176.
Qin, Y., Liu, Y., Yong, H., Liu, J., Zhang, X. & Liu,
J. 2019. Preparation and characterization of active and intelligent packaging
films based on cassava starch and anthocyanins from Lycium ruthenicum. International
Journal of Biological Macromolecules 134: 80-90.
Rahim, M.Z.A., Husin, N., Mohd Noor, M.A., Yet, Z.R. &
Ismail-Fitry, M.R. 2020. Screening of natural colours from various natural
resources as potential reusable visual indicators for monitoring food
freshness. Malaysian Journal of Analytical Sciences 24(2): 288-299.
Roy, S., Rhim, J-W. & Jaiswal, L. 2019. Bioactive
agar-based functional composite film incorporated with copper sulfide
nanoparticles. Food Hydrocolloid 93: 156-166.
Sanyang, M.L., Sapuan, S.M., Jawaid, M., Ishak, M.R. &
Sahari, J. 2015. Effect of plasticizer type and concentration on tensile,
thermal and barrier properties of biodegradable films based on sugar palm (Arenga
pinnata) starch. Polymers 7: 1106-1124.
Sohany, M., Mohamed Amin Tawakkal, I.S., Ariffin, S.H.,
Abdul Karim Shah, N.N. & Yusof, Y.A. 2021. Characterization of anthocyanin
associated purple sweet potato starch and peel-based ph indicator films. Foods 10(9): 2005.
Suratsawadee, A., Wangmo, L., Ratvijitvech, T. &
Siripinyanond, A. 2022. A spoilage indicator card based on distance-based color
change of paper impregnated with acid-base indicator for freshness monitoring
of shrimp. Microchemical Journal 175: 107110.
Vidana Gamage, G.C., Lim, Y.Y. & Choo, W.S. 2021.
Anthocyanins from Clitoria ternatea flower: Biosynthesis, extraction,
stability, antioxidant activity, and applications. Frontiers in Plant
Science 12: 3011.
Wu, D., Sun, D.W. & He, Y. 2014. Novel non-invasive
distribution measurement of texture profile analysis (TPA) in salmon fillet by
using visible and near infrared hyperspectral imaging. Food Chemistry 145:
417-426.
Xu, N., Shi, W., Wang, X. & Wang, Z. 2019. Effect of
ice water pretreatment on the quality of pacific white shrimps (Litopenaeus
vannamei). Food Science and Nutrition 7(2): 645-655.
Yan, J., Cui, R., Qin, Y., Li, L. & Yuan, M. 2021. A
pH indicator film based on chitosan and butterfly pudding extract for
monitoring fish freshness. International Journal of Biological
Macromolecules 177: 328-336.
Yong, H. & Liu, J. 2020. Recent advances in the preparation,
physical and functional properties, and applications of anthocyanins-based
active and intelligent packaging films. Food Packaging and Shelf Life 26:
100550.
Zahari, N.A.A.R., Chong, G.H., Abdullah, L.C. & Chua,
B.L. 2020. Ultrasonic-assisted extraction (UAE) process on thymol concentration
from Plectranthus amboinicus leaves: Kinetic modeling and optimization. Processes 8(3): 322.
Zhai, X., Li, Z., Zhang, J., Shi, J., Zou, X., Huang, X.,
Zhang, D., Sun, Y., Yang, Z., Holmes, M., Gong, Y. & Povey, M. 2018.
Natural biomaterial-based edible and pH-sensitive films combined with
electrochemical writing for intelligent food packaging. Journal of
Agricultural and Food Chemistry 66(48): 12836-12846.
Zhang, X., Liu, Y., Yong, H., Qin, Y., Liu, J. & Liu, J.
2020. Development of multifunctional food packaging films based on chitosan,
TiO2 nanoparticles and anthocyanin-rich black plum peel extract. Food
Hydrocolloids 94: 80-92.
*Pengarang
untuk surat-menyurat; email: ismailfitry@upm.edu.my
|